Journal article
A Broad-Spectrum α-Glucosidase of Glycoside Hydrolase Family 13 from Marinovum sp., a Member of the Roseobacter Clade
J Li, JWY Mui, BM da Silva, DEV Pires, DB Ascher, N Madiedo Soler, ED Goddard-Borger, SJ Williams
Applied Biochemistry and Biotechnology | SPRINGER | Published : 2024
Abstract
Glycoside hydrolases (GHs) are a diverse group of enzymes that catalyze the hydrolysis of glycosidic bonds. The Carbohydrate-Active enZymes (CAZy) classification organizes GHs into families based on sequence data and function, with fewer than 1% of the predicted proteins characterized biochemically. Consideration of genomic context can provide clues to infer possible enzyme activities for proteins of unknown function. We used the MultiGeneBLAST tool to discover a gene cluster in Marinovum sp., a member of the marine Roseobacter clade, that encodes homologues of enzymes belonging to the sulfoquinovose monooxygenase pathway for sulfosugar catabolism. This cluster lacks a gene encoding a classi..
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Grants
Awarded by Walter and Eliza Hall Institute of Medical Research
Funding Acknowledgements
S.J.W is supported by the Australian Research Council (DP210100233, DP210100235, DP240100126). D.B.A. is supported by an Investigator Grant from the National Health and Medical Research Council (NHMRC) of Australia (GNT1174405). E.D.G-B. acknowledges support from The Walter and Eliza Hall Institute of Medical Research, National Health and Medical Research Council of Australia (NHMRC) Ideas grant GNT2000517, the Australian Cancer Research Fund; and the Brian M. Davis Charitable Foundation Centenary Fellowship. JL thanks the China Scholarship Council for a PhD scholarship. BMdS is supported by Melbourne Research Scholarship. Phillip van der Peet is thanked for providing 6-P-PNPGlc.